1,25 (OH)2 Vitamin D ELISA Kit from IMMUNDIAGNOSTIK AG

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Product Name	1,25 (OH)2 Vitamin D ELISA Kit
Description	The assay utilizes of a competitive Enzyme-Immuno-Assay (EIA) technique with a selected monoclonal antibody recognizing 1,25-dihydroxy vitamin D. Standards, NSB (non-specific binding), controls and patient samples which are assayed for 1,25-dihydroxy vitamin D are incubated after the extraction step with the detection antibody. The pre-incubated solution is then transferred to the microplate coated with 1,25-dihydroxy vitamin D. During this incubation step, 1,25-dihydroxy vitamin D in the sample and a fixed amount of 1,25-dihydroxy vitamin D bound to the microtiter well compete for the binding of the detection antibodies. Then a peroxidase-conjugated anti-mouse antibody is added into each microplate well and a complex of 1,25-dihydroxy vitamin D - detection antibody - peroxidase conjugate is formed. Tetramethylbenzidine (TMB) is used as a peroxidase substrate. Finally, an acidic stop solution is added to terminate the reaction, whereby the color changes from blue to yellow. The intensity of the yellow color is inversely proportional to the concentration of 1,25-dihydroxy vitamin D. A dose response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standard. 1,25-dihydroxy vitamin D in the samples is determined from this curve. Range: 5.1-200 pg/ml
Size	48 tests
Concentration	n/a
Applications	n/a
Other Names	Vitamin D
Gene, Accession, CAS #	n/a
Catalog #	K 2112
Price	please inquire
Order / More Info	1,25 (OH)2 Vitamin D ELISA Kit from IMMUNDIAGNOSTIK AG
Product Specific References	n/a

IMMUNDIAGNOSTIK AG

IMMUNDIAGNOSTIK AG
Stubenwald Allee 8a
64625 Bensheim GERMANY
P: 49-6251701900
F: 49-6251849430

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