IDK® IDO activity ELISA from IMMUNDIAGNOSTIK AG
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| Product Name | IDK® IDO activity ELISA |
| Description | This assay is based on the method of competitive enzyme linkedimmunoassays.The sample preparation includes the addition of a derivatization reagent forderivatization of L-kynurenine and L-tryptophan. Afterwards, the treatedstandards, controls and samples are incubated in wells of two microtiter platescoated with(I) L-kynurenine-derivative (tracer) (red mark),(II) L-tryptophan-derivative (tracer) (yellow mark).Also, a polyclonal L-kynurenine antiserum and a polyclonal L-tryptophanantiserum is added respectively. During the incubation period the targetantigen in the sample competes with the tracer, immobilized on the wall of themicrotiter wells, for the binding of the polyclonal antibodies.In the second incubation step, a peroxidase-conjugated antibody is added toeach microtiter well to detect the polyclonal antibodies. After washing awaythe unbound components, tetramethylbenzidine (TMB) is added as aperoxidase substrate. Finally, the enzymatic reaction is terminated by an acidicstop solution. The color changes from blue to yellow and the absorbance ismeasured in the photometer at 450 nm. The intensity of the yellow color is inverse proportional to the target antigenconcentration in the sample. This means, high L-kynurenine or L-tryptophanconcentration in the sample reduces the concentration of tracer-boundantibodies and lowers the photometric signal. A dose response curve ofabsorbance unit (optical density, OD at 450 nm) vs. standard concentration isgenerated using the values obtained from the standards. L-kynurenine and Ltryptophanpresent in the patient samples are determined directly from thiscurves. |
| Size | 96 wells |
| Concentration | n/a |
| Applications | n/a |
| Other Names | IDO |
| Gene, Accession, CAS # | n/a |
| Catalog # | K 7726 |
| Price | please inquire |
| Order / More Info | IDK® IDO activity ELISA from IMMUNDIAGNOSTIK AG |
| Product Specific References | n/a |
IMMUNDIAGNOSTIK AG
IMMUNDIAGNOSTIK AG
Stubenwald Allee 8a
64625 Bensheim GERMANY
P: 49-6251701900
F: 49-6251849430
info@immundiagnostik.com
http://www.immundiagnostik.com
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