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Product Name | Vitamin K1/K2 HPLC Kit |
Description | The first step in the determination of vitamin K1 and K2 (MK-4 and MK-7) is the sample preparation. A precipitation reagent mixed with internal standard is added to the plasma or serum samples for separation of higher molecular weight substances. Elution solution is then added to the sample to transfer the vitamins K1 and K2 to the organic phase. After elution the organic phase is evaporated. The sample is then resuspended in mobile phase and subsequently measured in an isocratic HPLC-system. A post-column reduction reactor reduces vitamin K1 and K2 and enables the measurement of these vitamins with a fluorescence detector. The separation requires approx. 16.5 minutes for each run. Quantification is performed using the supplied plasma calibrator according to the internal standard method. |
Size | 100 tests |
Concentration | n/a |
Applications | n/a |
Other Names | Vitamin K1/K2 |
Gene, Accession, CAS # | n/a |
Catalog # | KC2402 |
Price | please inquire |
Order / More Info | Vitamin K1/K2 HPLC Kit from IMMUNDIAGNOSTIK AG |
Product Specific References | n/a |