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Product Name | IDKmonitor® Vedolizumab drug level ELISA |
Description | This ELISA is designed for the quantitative determination of free vedolizumab (therapeutic antibody against α4β7 integrin, e. g. ENTYVIO®) in EDTA plasma or serum samples. In a first incubation step, the free vedolizumab from the sample is bound to the specific monoclonal anti-vedolizumab antibody coated on the plate. To remove all unbound substances, a washing step is carried out. In a further incubation step, Peroxidase-labelled antibody is added. Tetramethylbenzidine (TMB) is used as a substrate for peroxidase. Finally, an acidic stop solution is added to terminate the reaction. The colour changes from blue to yellow. The intensity of the yellow colour is directly proportional to the concentration of free vedolizumab in the sample. A dose response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated, using the values obtained from the standard. Free vedolizumab, present in the patient samples, is determined directly from this curve. 18. Range: 15-1000 ng/ml |
Size | 96 wells |
Concentration | n/a |
Applications | n/a |
Other Names | Vedolizumab |
Gene, Accession, CAS # | n/a |
Catalog # | K 9658 |
Price | please inquire |
Order / More Info | IDKmonitor® Vedolizumab drug level ELISA from IMMUNDIAGNOSTIK AG |
Product Specific References | n/a |