MutaREAL® ApoE from IMMUNDIAGNOSTIK AG
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| Product Name | MutaREAL® ApoE |
| Description | For analysis of mutation ApoE*112 and *158 the potentially mutated region of ApoE geneis amplified by PCR (in a capillary by LightCycler®) using genomic DNA. Amplificationproducts are analyzed afterwards in a melting-point curve analysis with mutation specifichybridization probes detected at 670nm (F3-channel). The melting-point curve analysisallows a clear identification of wildtype, heterozygous or homozygous genotypes. ForPCR are primer used which flank the region around the potential mutation place andproduce an amplicon of 258 bp. In addition, the standard PCR contains for eachpolymorphism two sequence specific oligonucleotides (Hybridization Probes) which bindbetween both primers in proximity to the target DNA. The PCR step follows a genotypediscrimination through melting point curve analysis. One of the two Hybridization Probesbinds exactly within the range of mutation site.For ApoE*112 and *158 the Hybridization Probes are designed in this way that theirsequence fits exactly onto the CGC - sequence. Therefore, in the melting point curveanalysis the mutation peak arises earlier in presence of TGC-sequence because of theintroduced mismatch (= sequences are not 100 % homologous). In case of heterozygotegenotype two peaks are generated - one with lower temperature (TGC) and one withhigher temperature (CGC). |
| Size | 96 tests |
| Concentration | n/a |
| Applications | n/a |
| Other Names | Apo E |
| Gene, Accession, CAS # | n/a |
| Catalog # | KF290796 |
| Price | please inquire |
| Order / More Info | MutaREAL® ApoE from IMMUNDIAGNOSTIK AG |
| Product Specific References | n/a |
IMMUNDIAGNOSTIK AG
IMMUNDIAGNOSTIK AG
Stubenwald Allee 8a
64625 Bensheim GERMANY
P: 49-6251701900
F: 49-6251849430
info@immundiagnostik.com
http://www.immundiagnostik.com
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