IDK® Kynurenine ELISA from IMMUNDIAGNOSTIK AG
Search, find, compare suppliers for IDK® Kynurenine ELISA products.
| Edit | |
|---|---|
| Product Name | IDK® Kynurenine ELISA |
| Description | This assay is based on the method of competitive enzyme linkedimmunoassays. The sample preparation includes the addition of aderivatization reagent for L-kynurenine derivatization. Afterwards, the treatedsamples and a polyclonal L-kynurenine-antiserum are incubated in wells of amicrotiter plate coated with L-kynurenine-derivative (tracer).During the incubation period the target L-kynurenine in the sample competeswith the tracer, immobilized on the wall of the microtiter wells, for the bindingof the polyclonal antibodies. The L-kynurenine in the sample displaces theantibodies out of the binding to the tracer. Therefore the concentration of thetracer-bound antibody is inverse proportional to the kynurenine concentrationin the sample.During the second incubation step, a peroxidase-conjugated antibody is addedto each microtiter well to detect the anti-kynurenine antibodies. After washing away the unbound components, tetramethylbenzidine (TMB) is added as aperoxidase substrate. Finally, the enzymatic reaction is terminated by an acidic stop solution. The color changes from blue to yellow and the absorbance is measured in the photometer at 450 nm. The intensity of the yellow color is inverse proportional to the L-kynurenine concentration in the sample; this means, high L-kynurenine concentration in the sample reduces the concentration of tracer-bound antibodies and lowers the photometric signal. A dose response curve of absorbance unit (optical density, OD at 450 nm) vs. concentration is generated, using the values obtained from the standards. L-kynurenine, present in the patient samples is determined directly from this curve. Range: 0.1 - 10 µM |
| Size | 96 wells |
| Concentration | n/a |
| Applications | n/a |
| Other Names | Kynurenine |
| Gene, Accession, CAS # | n/a |
| Catalog # | K 7728 |
| Price | please inquire |
| Order / More Info | IDK® Kynurenine ELISA from IMMUNDIAGNOSTIK AG |
| Product Specific References | n/a |
IMMUNDIAGNOSTIK AG
IMMUNDIAGNOSTIK AG
Stubenwald Allee 8a
64625 Bensheim GERMANY
P: 49-6251701900
F: 49-6251849430
info@immundiagnostik.com
http://www.immundiagnostik.com
Profile of IMMUNDIAGNOSTIK AG