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Product Name | IDK® α1 -Antitrypsin Clearance ELISA |
Description | This ELISA is designed for the quantitative determination of α1-Antitrypsin in serum, plasma and stool. The assay utilises the sandwich technique with two selected polyclonal antibodies that bind to human α1-antitrypsin. Standards, controls and prediluted samples which are assayed for human α1- antitrypsin are added into the wells of a microtiter plate coated with a high affine polyclonal anti-human α1-antitrypsin antibody. During the first incubation step, α1- antitrypsin is bound by the immobilised antibody. Then a peroxidase-conjugated polyclonal anti-human α1-antitrypsin antibody is added into each microtiter well and a sandwich of capture antibody - human α1-antitrypsin - peroxidase-conjugate is formed. Tetramethylbenzidine (TMB) is used as peroxidase substrate. Finally, an acidic stop solution is added to terminate the reaction. The colour changes from blue to yellow. The intensity of the yellow colour is directly proportional to the concentration of α1-antitrypsin. A dose response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated, using the values obtained from the standard. α1-antitrypsin present in the samples is determined directly from this curve. Range: 3.3-90 µg/l |
Size | 96 wells |
Concentration | n/a |
Applications | n/a |
Other Names | Alpha 1 Antitrypsin Clearance |
Gene, Accession, CAS # | n/a |
Catalog # | K 6752 |
Price | please inquire |
Order / More Info | IDK® α1 -Antitrypsin Clearance ELISA from IMMUNDIAGNOSTIK AG |
Product Specific References | n/a |