IDK® DAO ELISA (1-point calibration) from IMMUNDIAGNOSTIK AG
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| Product Name | IDK® DAO ELISA (1-point calibration) |
| Description | The assay utilizes the ``sandwich'' technique with two polyclonal antibodies against recombinant DAO. Calibrator, controls and diluted samples which are assayed for DAO are added into the wells of a micro plate coated with polyclonal rabbit anti- DAO antibody. During the first incubation step, DAO is bound by the immobilized primary antibody. Then a biotinylated polyclonal anti-DAO antibody, is added into each microtiter well. In the next step, the streptavidin-POD-conjugate is added and a ``sandwich'' of 1st antibody - DAO - biotinylated antibody - streptavidin-POD-conjugate is formed. Tetramethylbenzidine (TMB) is used as peroxidase substrate. Finally, an acidic stop solution is added to terminate the reaction. The colour changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of DAO. DAO, present in the patients` samples, ist quantified by referring the optical density (OD) of the samples to a lot-dependent master calibration curve. This is done by using calibrator that is run with each test. |
| Size | 96 wells |
| Concentration | n/a |
| Applications | n/a |
| Other Names | DAO |
| Gene, Accession, CAS # | n/a |
| Catalog # | K 8510 |
| Price | please inquire |
| Order / More Info | IDK® DAO ELISA (1-point calibration) from IMMUNDIAGNOSTIK AG |
| Product Specific References | n/a |
IMMUNDIAGNOSTIK AG
IMMUNDIAGNOSTIK AG
Stubenwald Allee 8a
64625 Bensheim GERMANY
P: 49-6251701900
F: 49-6251849430
info@immundiagnostik.com
http://www.immundiagnostik.com
Profile of IMMUNDIAGNOSTIK AG