Histamine ELISA from IMMUNDIAGNOSTIK AG

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Product NameHistamine ELISA
DescriptionThis assay is based on the method of competitive enzyme linked immunoassays. The sample preparation includes the addition of aderivatization reagent for histamine derivatization. Afterwards, the treatedsamples and a peroxidase-conjugated polyclonal histamine antibody areincubated in wells of a microtiter plate coated with histamine derivative(tracer). During the incubation period, the target histamine in the samplecompetes with the tracer, immobilized on the wall of the microtiter wells, forthe binding of the polyclonal antibodies.After washing away the unbound components tetramethylbenzidine (TMB) isadded as a peroxidase substrate. Finally, the enzymatic reaction is terminatedby an acidic stop solution. The color changes from blue to yellow, and theabsorbance is measured in a photometer at 450 nm. The intensity of the yellowcolor is inverse proportional to the histamine concentration in the sample; thismeans, high histamine concentration in the sample reduces the concentrationof tracer-bound antibody and lowers the photometric signal.A dose response curve of the absorbance unit (optical density, OD at 450 nm)vs. concentration is generated, using the values obtained from the standards.Histamine, present in the patient samples, is determined directly from thiscurve. Range: 0.3-25 ng/ml
Size96 wells
Concentrationn/a
Applicationsn/a
Other NamesHistamine
Gene, Accession, CAS #n/a
Catalog #K 8212
Priceplease inquire
Order / More InfoHistamine ELISA from IMMUNDIAGNOSTIK AG
Product Specific Referencesn/a
IMMUNDIAGNOSTIK AG
IMMUNDIAGNOSTIK AG
IMMUNDIAGNOSTIK AG
Stubenwald Allee 8a
64625 Bensheim GERMANY
P: 49-6251701900
F: 49-6251849430

info@immundiagnostik.com

http://www.immundiagnostik.com

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