Immunoglobulin E ELISA Kit from IMMUNDIAGNOSTIK AG

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Product NameImmunoglobulin E ELISA Kit
DescriptionThis ELISA is designed for the quantitative determination of IgE. In a first incubation step, the IgE in the samples is bound to polyclonal antibodies (in excess), which are immobilised to the surface of the microtiter wells. To remove all unbound substances, a washing step is carried out. In a second incubation step, a peroxidase-labelled anti-IgE (POD-Antibody) antibody (polyclonal) is added. After another washing step, to remove all unbound substances, the solid phase is incubated with the substrate, Tetramethylbenzidine (TMB). An acidic stop solution is then added to stop the reaction. The colour converts from blue to yellow. The intensity of the yellow colour is directly proportional to the concentration of IgE. A dose response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated, using the values obtained from the standard. IgE, present in the patient samples, is determined directly from this curve. Range: 0,62-40 kU/l
Size96 wells
Concentrationn/a
Applicationsn/a
Other NamesImmunoglobulin E
Gene, Accession, CAS #n/a
Catalog #K 6511
Priceplease inquire
Order / More InfoImmunoglobulin E ELISA Kit from IMMUNDIAGNOSTIK AG
Product Specific Referencesn/a
IMMUNDIAGNOSTIK AG
IMMUNDIAGNOSTIK AG
IMMUNDIAGNOSTIK AG
Stubenwald Allee 8a
64625 Bensheim GERMANY
P: 49-6251701900
F: 49-6251849430

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