total sRANKL (human) ELISA Kit from IMMUNDIAGNOSTIK AG
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| Product Name | total sRANKL (human) ELISA Kit |
| Description | The assay utilizes the two-site sandwich technique with two selected antibodies that bind to human sRANKL and OPG. Assay standards, controls, prediluted patient samples containing human sRANKL and the OPG solution are added to wells of microplate coated with a high affine polyclonal anti-human OPG antibody. After the first incubation period, sRANKL is bound to the OPG and the antibody immobilized on the wall of microtiter wells. Then a biotinylated monoclonal anti-human sRANKL antibody is added to each microtiter well and a sandwich of capture antibody - human OPG - sRANKL - streptavidin (peroxidase-labeled) is formed. For quantification, a streptavidin horseradish-peroxidase conjugate is added, which specifically binds to biotin. Tetramethylbenzidine (TMB) is used as a substrate for peroxidase. Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of total sRANKL. A dose response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated, using the values obtained from the standard. total sRANKL present in the patient samples, is determined directly from this curve. Range: 370-30000 pg/ml |
| Size | 96 wells |
| Concentration | n/a |
| Applications | n/a |
| Other Names | sRANKL |
| Gene, Accession, CAS # | n/a |
| Catalog # | K 1016 |
| Price | please inquire |
| Order / More Info | total sRANKL (human) ELISA Kit from IMMUNDIAGNOSTIK AG |
| Product Specific References | n/a |
IMMUNDIAGNOSTIK AG
IMMUNDIAGNOSTIK AG
Stubenwald Allee 8a
64625 Bensheim GERMANY
P: 49-6251701900
F: 49-6251849430
info@immundiagnostik.com
http://www.immundiagnostik.com
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