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Product Name | IDKmonitor® Adalimumab total ADA ELISA |
Description | This ELISA is designed for the quantitative determination of antibodies against TNFα blocker adalimumab (e. g. HUMIRA®). During sample preparation, the anti-drug antibodies (ADA) are separated from the therapeutic antibody in order to acquire free ADA. By adding the peroxidase conjugate (peroxidase labelled therapeutic antibody) and the tracer (biotinylated therapeutic antibody), the unmarked therapeutic antibodies are replaced and the marked antibodies can form a complex with the ADA. This complex binds via biotin to the streptavidin coated microtiter plate. It is detected via the peroxidase conjugate with the peroxidase converting the substrate TMB to a blue product. The enzymatic reaction is stopped by adding an acidic solution. The samples convert from blue to yellow. The colour change should be measured in a photometer at 450 nm. The interpretation is made using the cut-off control. |
Size | 96 wells |
Concentration | n/a |
Applications | n/a |
Other Names | Adalimumab |
Gene, Accession, CAS # | n/a |
Catalog # | K 9651 |
Price | please inquire |
Order / More Info | IDKmonitor® Adalimumab total ADA ELISA from IMMUNDIAGNOSTIK AG |
Product Specific References | n/a |