MutaREAL® DPD from IMMUNDIAGNOSTIK AG
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| Product Name | MutaREAL® DPD |
| Description | MutaREAL® DPD real time PCR Kit contains specific primers and additional material for the detection of DPD*2A (exon 14-skipping) polymorphism in the dihydropyrimidine-dehydrogenase gene (DPD) with the LightCycler® (Roche). The variable area of the DPD gene is amplified by PCR using LightCycler®-capillaries and genomic DNA-template. The amplification product is subsequently analyzed by subjecting to melting curve including specifc hybridisation probes. This results in the final identification of genotype variants G/G, G/A or A/A. The specific primers used in the kit flank the variable area of DPD gene and generate an amplificate of 269 bp. Genotyping is performed by subsequent melting curve analysis of arised amplificates. This is due to the different melting points of the complexes formed by DNA template and ``SNP-probes''. The included ``SNP-probe'' is 100% homologous to the exon-14-skipping wildtype (G/G) variant. Therefore the hybridisation probe needs a higher temperature for complex-dissociation from G-allel than from the A-allel (containing a mismatch destabi-lizing the complex). Consequently, the melting curve for A-allel will be generated earlier (respectively at lower temperature). Samples with heterozygous genotype generate both peaks at different temperatures during the melting curve process. |
| Size | 96 tests |
| Concentration | n/a |
| Applications | n/a |
| Other Names | DPD |
| Gene, Accession, CAS # | n/a |
| Catalog # | KF291196 |
| Price | please inquire |
| Order / More Info | MutaREAL® DPD from IMMUNDIAGNOSTIK AG |
| Product Specific References | n/a |
IMMUNDIAGNOSTIK AG
IMMUNDIAGNOSTIK AG
Stubenwald Allee 8a
64625 Bensheim GERMANY
P: 49-6251701900
F: 49-6251849430
info@immundiagnostik.com
http://www.immundiagnostik.com
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