IDK® EDN ELISA from IMMUNDIAGNOSTIK AG
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| Product Name | IDK® EDN ELISA |
| Description | The assay utilizes the two-site sandwich ELISA technique with two selected antibodies (monoclonal and polyclonal) that bind to human EDN. Calibrator, controls and diluted patient samples which are assayed for EDN are added to wells of microplate coated with a high affine monoclonal anti-human EDN antibody. During the first incubation step, EDN in the samples is bound by the immobilized antibody. Then a peroxidase-labeled conjugate is added to each well and the following complex is formed: capture antibody - human EDN - peroxidase conjugate. Tetramethylbenzidine is used as a substrate for peroxidase. Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the EDN concentration of the sample. Samples are quantified by referring their optical density to a lot-dependent master calibration curve and the use of the calibrator that is run with each test. Range: 0.25 - 16 ng/ml |
| Size | 96 wells |
| Concentration | n/a |
| Applications | n/a |
| Other Names | EDN |
| Gene, Accession, CAS # | n/a |
| Catalog # | K 6821 |
| Price | please inquire |
| Order / More Info | IDK® EDN ELISA from IMMUNDIAGNOSTIK AG |
| Product Specific References | n/a |
IMMUNDIAGNOSTIK AG
IMMUNDIAGNOSTIK AG
Stubenwald Allee 8a
64625 Bensheim GERMANY
P: 49-6251701900
F: 49-6251849430
info@immundiagnostik.com
http://www.immundiagnostik.com
Profile of IMMUNDIAGNOSTIK AG