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Product Name | ox-LDL/MDA Adduct ELISA |
Description | This ELISA is designed for the quantitative determination of ox-LDL/MDA adducts. This assay is a sandwich ELISA for the direct measurement of ox-LDL in human EDTA plasma and serum. Standards, controls and samples containing human ox-LDL are added to wells of microplate coated with high affinity antibodies. During the first incubation period, the antibodies immobilised on the wall of the microtiter wells capture the antigen in the patient samples. After washing away the unbound components from samples, a peroxidase-conjugated antibody is added to each microtiter well. Tetramethylbenzidine (TMB) is used as a substrate for peroxidase. Finally, an acidic stop solution is added to terminate the reaction. The intensity of the yellow colour is directly proportional to the ox-LDL concentration of sample. A dose response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated, using the values obtained from the standard. Ox-LDL, present in the patient samples, is determined directly from this curve. Range: 9 - 250 ng/ml |
Size | 96 wells |
Concentration | n/a |
Applications | n/a |
Other Names | ox-LDL/MDA |
Gene, Accession, CAS # | n/a |
Catalog # | K 7810 |
Price | please inquire |
Order / More Info | ox-LDL/MDA Adduct ELISA from IMMUNDIAGNOSTIK AG |
Product Specific References | n/a |