Nitrotyrosine ELISA from IMMUNDIAGNOSTIK AG
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| Product Name | Nitrotyrosine ELISA |
| Description | This ELISA is designed for the quantitative determination of nitrotyrosine. It utilizes the ``sandwich'' technique with two polyclonal antibodies against nitrated proteins. Standards, controls and diluted samples which are assayed for nitrotyrosine are added into the wells of a microtiter plate coated with polyclonal anti-nitrotyrosine antibody. During the first incubation step, nitrated proteins are bound by the immobilized primary antibody. Then a peroxidase-conjugated polyclonal anti-nitrotyrosine antibody is added into each microtiter well and a ``sandwich'' of primary antibody - nitrated protein - peroxidase-conjugate is formed. Tetramethylbenzidine is used as peroxidase substrate. Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is Manual Nitrotyrosine ELISA 22 directly proportional to the concentration of nitrotyrosine. A dose response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated, using the values obtained from the standards. Nitrotyrosine, present in the patient samples, is determined directly from this curve. Range: 55.6-1500 nM |
| Size | 96 wells |
| Concentration | n/a |
| Applications | n/a |
| Other Names | Nitrotyrosine |
| Gene, Accession, CAS # | n/a |
| Catalog # | K 7824 |
| Price | please inquire |
| Order / More Info | Nitrotyrosine ELISA from IMMUNDIAGNOSTIK AG |
| Product Specific References | n/a |
IMMUNDIAGNOSTIK AG
IMMUNDIAGNOSTIK AG
Stubenwald Allee 8a
64625 Bensheim GERMANY
P: 49-6251701900
F: 49-6251849430
info@immundiagnostik.com
http://www.immundiagnostik.com
Profile of IMMUNDIAGNOSTIK AG