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Product Name | MutaPLATE® VDR3/Bsm1 (TM) |
Description | MutaPLATE® VDR (TM) real time PCR Kit contains specific primers and additionalmaterial for the detection of the BsmI polymorphism. The variable region of the gene(VDR) is amplified by PCR using genomic DNA template as template. The specificprimers used in the kit flank the variable area and generate an amplificate.The standard PCR contains additionally two sequence specific oligonucleotidesmarked with fluorescence dye (TaqMan probes). Both probes bind at the respectiveamplificated target-DNA which includes the single nucleotide polymorphism (SNP). Due tothis, a fluorescence signal is generated and detected by the optical unit of the used realtime PCR instrument. The TaqMan probe for the C-allele (wildtype) is marked with FAM(510 - 530 nm, green) and the TaqMan probe for the T-allele (mutation) is marked withYAK (555 - 560 nm, yellow). The following three discriminations for each of the polymorphisms are possible:BsmI1. Homozygous C/C:Increase of the fluorescent signal from the FAM labeled TaqMan probe, noincrease of the fluorescent signal from the YAK labeled TaqMan probe.2. Heterozygous C/T:Increase of the fluorescent signal from the FAM labeled TaqMan probe andincrease of the fluorescent signal from the YAK labeled TaqMan probe.3. Homozygous T/T:No increase of the fluorescent signal from the FAM labeled TaqMan probe,increase of the fluorescent signal from the YAK labeled TaqMan probe. |
Size | 32 tests |
Concentration | n/a |
Applications | n/a |
Other Names | VDR2-Bsml |
Gene, Accession, CAS # | n/a |
Catalog # | KF1900432 |
Price | please inquire |
Order / More Info | MutaPLATE® VDR3/Bsm1 (TM) from IMMUNDIAGNOSTIK AG |
Product Specific References | n/a |