MutaREAL® TPMT from IMMUNDIAGNOSTIK AG
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| Product Name | MutaREAL® TPMT |
| Description | For analysis of mutation G238C (TPMT*2), G460A (TPMT*3A, *3B) and A719G(TPMT*3A, *3C) the potentially mutated region of TPMT gene is amplified by PCR(in a capillary by LightCycler®) using genomic DNA. Amplification products areanalyzed afterwards in a melting-point curve analysis with mutation-specifichybridization probes. The melting-point curve analysis allows a clear identification ofwildtype, heterozygous or homozygous genotypes. For G238C (*2), primers areused which flank the region around the potential mutation place and produce anamplicon of 197 bp. For G460A (*3A, *3B) and A719G (*3A, *3C), two primer pairsare used generating amplicons of 159 bp and respectively 177 bp. In addition, thestandard PCR contains for each polymorphism two sequence-specific oligonucleotides(Hybridization Probes) which bind between both primers in proximity tothe target DNA. The PCR step follows a genotype discrimination through meltingpointcurve analysis. One of the two Hybridization Probes binds exactly within therange of mutation site. For mutation G238C (*2) and G460A (*3A, *3B) theHybridization Probes are designed in this way that their sequence fits exactly ontothe wildtype- constellation. Therefore, in the melting-point curve analysis the mutation peak arises earlier because of the mismatch introduced with the mutation (=sequences are not 100% homologous). In case of heterozygote genotype two peaksare generated - one with lower temperature (mutation) and one with highertemperature (wildtype). For mutation A719G (*3A, *3C) the Hybridization Probesare designed in this way that their sequence fits exactly onto the mutationconstellation.Therefore, in the melting curve analysis the wildtype peak arises earlierthan the mutation peak. |
| Size | 96 tests |
| Concentration | n/a |
| Applications | n/a |
| Other Names | TPMT |
| Gene, Accession, CAS # | n/a |
| Catalog # | KF291332 |
| Price | please inquire |
| Order / More Info | MutaREAL® TPMT from IMMUNDIAGNOSTIK AG |
| Product Specific References | n/a |
IMMUNDIAGNOSTIK AG
IMMUNDIAGNOSTIK AG
Stubenwald Allee 8a
64625 Bensheim GERMANY
P: 49-6251701900
F: 49-6251849430
info@immundiagnostik.com
http://www.immundiagnostik.com
Profile of IMMUNDIAGNOSTIK AG