MutaREAL® Laktase from IMMUNDIAGNOSTIK AG
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| Product Name | MutaREAL® Laktase |
| Description | To analyse the -13910 T/C polymorphism, the variable region of the gene is amplifiedusing real time PCR (e.g. the LightCycler® system). Genomic DNA serves as a templatefor this amplification. Using this amplicon a melting curve analysis is performed thatincorporates specific FRET-hybridisation probes detected at 670 nm. This melting curveanalysis facilitates the distinct identification of the -13910T or -13910C polymorphismgenotype. For the real time PCR reaction a primer is used that flanks the variable region inthe gene and produces a fragment. In addition two probes (fluorescent labeled sequencespecific oligonucleotides; anchor and reporter probe) are incorporated into the PCRreaction mix. Both bind the target DNA in close proximity in a region between the primerpair. Upon this binding a fluorescent signal is generated that is detected by the optical unitof the real time PCR instrument (e.g. the LightCycler®).For the genotyping analysis a melting curve is recorded after the amplification reaction.This is achieved by constantly increasing the temperature. The reporter probe containedin this kit is a perfect match for the wildtype allele and hence requires a highertemperature to be melted from wildtype DNA than from mutated DNA, as here amismatch occurs. The sequence of the reporter probe matches the mutated DNA (-13910C) perfectly. The hybrids of probe and target DNA will melt at lower temperatures ifthe DNA contains the-13910T variant. In case of a heterozygous sample (only one of thetwo alleles carries the -13910C mutation), two peaks will be generated, one at the lowertemperature (melting point of the wildtype) and one at the higher temperature (meltingpoint of the perfectly matched mutation). |
| Size | 96 tests |
| Concentration | n/a |
| Applications | n/a |
| Other Names | Laktase |
| Gene, Accession, CAS # | n/a |
| Catalog # | KF2907196 |
| Price | please inquire |
| Order / More Info | MutaREAL® Laktase from IMMUNDIAGNOSTIK AG |
| Product Specific References | n/a |
IMMUNDIAGNOSTIK AG
IMMUNDIAGNOSTIK AG
Stubenwald Allee 8a
64625 Bensheim GERMANY
P: 49-6251701900
F: 49-6251849430
info@immundiagnostik.com
http://www.immundiagnostik.com
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