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Product Name | IDK® MPO ELISA |
Description | This ELISA is designed for the quantitative determination of myeloperoxidase (MPO) in urine and stool. In a first incubation step, the myeloperoxidase in the samples is bound to an available excess of antibodies against myeloperoxidase, which are immobilised to the surface of the microtiter plates. To remove all unbound substances, a washing step is carried out. In a second incubation step, a peroxidase-labelled antibody against MPO is added. After another washing step, to remove all unbound substances, the solid phase is incubated with the substrate, tetramethylbenzidine (TMB). An acidic stop solution is then added to stop the reaction. The colour converts from blue to yellow. The intensity of the yellow colour is directly proportional to the concentration of MPO in the sample. A dose response curve of the absorbance unit (optical density, OD) vs. concentration is generated, using results obtained from the calibrators. MPO, present in the patient samples, is determined directly from this curve. Range: 1.9-30 ng/ml |
Size | 96 wells |
Concentration | n/a |
Applications | n/a |
Other Names | MPO |
Gene, Accession, CAS # | n/a |
Catalog # | K 6630 |
Price | please inquire |
Order / More Info | IDK® MPO ELISA from IMMUNDIAGNOSTIK AG |
Product Specific References | n/a |