IDK® DAO ELISA from IMMUNDIAGNOSTIK AG
Search, find, compare suppliers for IDK® DAO ELISA products.
| Edit | |
|---|---|
| Product Name | IDK® DAO ELISA |
| Description | This ELISA is designed for the quantitative determination of DAO in serum and dried blood spots. The assay utilises the sandwich technique with two polyclonal antibod ies against recombinant DAO. Standards, controls and prepared samples which are assayed for DAO are added into the wells of a micro plate coated with polyclonal rabbit anti-DAO antibody. During the first incubation step, DAO is bound by the immobilised primary antibody. Then a biotinylated polyclonal anti-DAO antibody is added into each microtiter well. In the next step, the streptavidin peroxidase conjugate is added and a ``sandwich''of 1st antibody - DAO - biotinylated antibody - streptavidin peroxidase conjugate is formed. Tetramethylbenzidine (TMB) is used as peroxidase substrate. Finally, an acidic stop solution is added to terminate the reaction. The colour changes from blue to yellow. The intensity of the yellow colour is directly proportional to the concentration of DAO. A dose response curve of the absorbance unit (optical density, OD at 450nm) vs. concentration is generated, using the values obtained from the standard. DAO, present in the patient samples, is determined directly from this curve. |
| Size | 96 wells |
| Concentration | n/a |
| Applications | n/a |
| Other Names | DAO |
| Gene, Accession, CAS # | n/a |
| Catalog # | K 8500 |
| Price | please inquire |
| Order / More Info | IDK® DAO ELISA from IMMUNDIAGNOSTIK AG |
| Product Specific References | n/a |
IMMUNDIAGNOSTIK AG
IMMUNDIAGNOSTIK AG
Stubenwald Allee 8a
64625 Bensheim GERMANY
P: 49-6251701900
F: 49-6251849430
info@immundiagnostik.com
http://www.immundiagnostik.com
Profile of IMMUNDIAGNOSTIK AG