Mouse/Rat Osteoprotegerin ELISA Kit, from IMMUNDIAGNOSTIK AG
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| Product Name | Mouse/Rat Osteoprotegerin ELISA Kit, |
| Description | This sandwich-type ELISA is an assay for the direct determination of OPG inserum, plasma and urine. In this assay two highly specific antibodies againstOPG are used. The capture antibody is attached to the wells of the microtiterplate, the detection antibody is labeled with biotin.In a first incubation step the samples and the biotinylated antibody againstOPG react with the coated capture antibody on the microtiter plate. Asandwich-type complex is formed consisting of the binding antibody on theplate, OPG and the biotinylated detection antibody. To remove all unspecificbound substances a washing step is carried out.In a second step streptavidin - peroxidase is added which reacts with thedetection antibody. After another washing step, the solid phase is incubatedwith the substrate, TMB. An acidic stopping solution is subsequently added.The blue color changes to yellow. The intensity of the yellow color is directlyproportional to the concentration of OPG in the sample.A dose - response curve of the absorbance units at 450 nm versusconcentration is generated. OPG in the samples is determined directly fromthis calibration curve.a. Range: 62.5-4000 pg/ml |
| Size | 96 wells |
| Concentration | n/a |
| Applications | RUO |
| Other Names | Osteoprotegerin |
| Gene, Accession, CAS # | n/a |
| Catalog # | KR1020 |
| Price | please inquire |
| Order / More Info | Mouse/Rat Osteoprotegerin ELISA Kit, from IMMUNDIAGNOSTIK AG |
| Product Specific References | n/a |
IMMUNDIAGNOSTIK AG
IMMUNDIAGNOSTIK AG
Stubenwald Allee 8a
64625 Bensheim GERMANY
P: 49-6251701900
F: 49-6251849430
info@immundiagnostik.com
http://www.immundiagnostik.com
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