IDK® EDN ELISA from IMMUNDIAGNOSTIK AG
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| Product Name | IDK® EDN ELISA |
| Description | The assay utilizes the two-site sandwich ELISA technique with two selected antibodies (monoclonal and polyclonal) that bind to human EDN. Assay standards, controls and prediluted patient samples containing human EDN are added to wells of microplate that was coated with a high affine monoclonal anti-human EDN antibody. After the first incubation period, antibody immobilized on the wall of microtiter wells captures human EDN in the sample. Then a peroxidase-conjugated rabbit polyclonal anti-human EDN antibody is added to each microtiter well and a sandwich of capture antibody - human EDN - Peroxidase-conjugate is formed. Tetramethylbenzidine is used as a substrate for peroxidase. Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of EDN. A dose response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated, using the values obtained from the standard. EDN present in the patient samples, is determined directly from this curve. Range: 0.25-16 ng/ml |
| Size | 96 wells |
| Concentration | n/a |
| Applications | n/a |
| Other Names | EDN |
| Gene, Accession, CAS # | n/a |
| Catalog # | K 6811 |
| Price | please inquire |
| Order / More Info | IDK® EDN ELISA from IMMUNDIAGNOSTIK AG |
| Product Specific References | n/a |
IMMUNDIAGNOSTIK AG
IMMUNDIAGNOSTIK AG
Stubenwald Allee 8a
64625 Bensheim GERMANY
P: 49-6251701900
F: 49-6251849430
info@immundiagnostik.com
http://www.immundiagnostik.com
Profile of IMMUNDIAGNOSTIK AG