IDK® kynurenic acid ELISACE from IMMUNDIAGNOSTIK AG
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| Product Name | IDK® kynurenic acid ELISACE |
| Description | The assay is based on the method of competitive enzyme linkedimmunoassays. Samples, standards and controls are incubated in wells of amicrotiter plate coated with kynurenic acid (antigen), together with aperoxidase labeled polyclonal anti-kynurenic acid antibody. During theincubation period the free target antigen in the sample competes with theantigen immobilized on the wall of the microtiter wells for the binding of theperoxidase-labeled polyclonal antibodies.After a washing step to remove the unbound components, the peroxidasesubstrate tetramethylbenzidine (TMB) is added. Finally, the enzymatic reactionis terminated by an acidic stop solution. The color changes from blue to yellowand the absorbance is measured in the photometer at 450 nm. The intensity ofthe yellow color is inverse proportional to the kynurenic acid concentration inthe sample; this means, high antigen concentration in the sample reduces theconcentration of antibodies bound to the antigen on the plate and lowers thephotometric signal. A dose response curve of absorbance unit (optical density,OD at 450 nm) vs. concentration is generated using the values obtained fromthe standards. Kynurenic acid present in the patient samples is determineddirectly from this curve. Range: 1.5-150 µmol/l |
| Size | 96 wells |
| Concentration | n/a |
| Applications | n/a |
| Other Names | KynA |
| Gene, Accession, CAS # | n/a |
| Catalog # | K 7735 |
| Price | please inquire |
| Order / More Info | IDK® kynurenic acid ELISACE from IMMUNDIAGNOSTIK AG |
| Product Specific References | n/a |
IMMUNDIAGNOSTIK AG
IMMUNDIAGNOSTIK AG
Stubenwald Allee 8a
64625 Bensheim GERMANY
P: 49-6251701900
F: 49-6251849430
info@immundiagnostik.com
http://www.immundiagnostik.com
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