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Product Name | MutaPLATE® Laktase (TM) |
Description | MutaPLATE® Laktase (TM) real time PCR Kit contains specific primers andadditional material for the detection of the T/C (-13910) polymorphism in the regulatoryregion of the lactase phlorizin hydrolase gene. The variable area of the regulatory regionfrom lactase gene is amplified by PCR using genomic DNA-template. The specificprimers used in the kit flank the variable area of lactase gene (LCT) and generate anamplificate of 300 bp using Taq-Man technology.The standard PCR contains additionally two sequence specific oligonucleotidesmarked with fluorescence dye (TaqMan probes). Both probes bind at the amplificatedtarget-DNA which includes the single nucleotide polymorphism (SNP). Due to this, afluorescence signal is generated and detected by the optical unit of the used real timePCR instrument. The TaqMan probe for the C-allele (mutation) is marked with FAM (510nm, green) and the TaqMan probe for the T-allele (wildtype) is marked with YAK (555 nm,yellow).The following three discriminations are possible:1. Homozygous C/C:Increase of the fluorescent signal from the FAM labeled TaqMan probe, noincrease of the fluorescent signal from the YAK labeled TaqMan probe.2. Heterozygous C/T:Increase of the fluorescent signal from the FAM labeled TaqMan probe andincrease of the fluorescent signal from the YAK labeled TaqMan probe.3. Homozygous T/T:No increase of the fluorescent signal from the FAM labeled TaqMan probe,increase of the fluorescent signal from the YAK labeled TaqMan probe. |
Size | 96 tests |
Concentration | n/a |
Applications | n/a |
Other Names | Laktase |
Gene, Accession, CAS # | n/a |
Catalog # | KF1907296 |
Price | please inquire |
Order / More Info | MutaPLATE® Laktase (TM) from IMMUNDIAGNOSTIK AG |
Product Specific References | n/a |