SpyCatcher003 Protein from KERAFAST, INC.

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Product NameSpyCatcher003 Protein
DescriptionSource: Recombinant expression in E. coli. Purity: >95%. Purified from Ni-NTA affinity chromatography and then by size exclusion chromatography. MW: 15,597.7 Da (SpyCatcher003) 15,613.7 Da (SpyCatcher003, S49C); 44,867.78 Da (SpyTag003-MBP)
Size0.5mg
Concentrationn/a
Applicationsn/a
Other Namesn/a
Gene, Accession, CAS #Accession: JQ478411.1
Catalog #EOX003
Price$66
Order / More InfoSpyCatcher003 Protein from KERAFAST, INC.
Product Specific References
  1. Keeble AH, Turkki P, Stokes S, Khairil Anuar INA, Rahikainen R, Hytonen VP, Howarth M. Approaching infinite affinity through engineering of peptide-protein interaction. Proc Natl Acad Sci U S A. 2019 Dec 10;116(52):26523-26533.
  2. Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, Howarth M. Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7.
  3. Veggiani G., Zakeri B., Howarth M. Superglue from Bacteria: Unbreakable Bridges for Protein Nanotechnology. Trends in Biotechnology 2014 Oct;32(10):506-12.
  4. Schoene C, Fierer JO, Bennett SP, Howarth M. SpyTag/SpyCatcher Cyclization Confers Resilience to Boiling on a Mesophilic Enzyme. Angewandte Chemie. 2014 Jun 10;53(24):6101-4.
  5. Fierer JO, Veggiani G, Howarth M. SpyLigase peptide-peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture. Proc Natl Acad Sci U S A. 2014 Apr 1;111(13):E1176-81.
  6. Li L, Fierer JO, Rapoport TA, Howarth M. Structural analysis and optimization of the covalent association between SpyCatcher and a peptide tag. Journal of Molecular Biology. 2014 Jan 23;426(2):309-17.
  7. Min, Duyoung; Arbing, Mark A; Jefferson, Robert E; Bowie, James U. A simple DNA handle attachment method for single molecule mechanical manipulation experiments.Protein Sci. 2016 01;25(8):1535-44 View Article
  8. Dovala, Dustin; Sawyer, William S; Rath, Christopher M; Metzger, Louis E. Rapid analysis of protein expression and solubility with the SpyTag-SpyCatcher system.Protein Expr Purif. 2016 01;117:44-51 View Article
  9. Alves, Nathan J; Turner, Kendrick B; Daniele, Michael A; Oh, Eunkeu; Medintz, Igor L; Walper, Scott A. Bacterial Nanobioreactors--Directing Enzyme Packaging into Bacterial Outer Membrane Vesicles.ACS Appl Mater Interfaces. 2015 11 11;7(44):24963-72 View Article
  10. Reddington, Samuel C; Howarth, Mark. Secrets of a covalent interaction for biomaterials and biotechnology: SpyTag and SpyCatcher.Curr Opin Chem Biol. 2015 12;29:94-9 View Article
  11. Janitzek, Christoph M; Matondo, Sungwa; Thrane, Susan; Nielsen, Morten A; Kavishe, Reginald; Mwakalinga, Steve B; Theander, Thor G; Salanti, Ali; Sander, Adam F. Bacterial superglue generates a full-length circumsporozoite protein virus-like particle vaccine capable of inducing high and durable antibody responses.Malar J. 2016 11 08;15(1):545 View Article
  12. Si, Meng; Xu, Qing; Jiang, Ling; Huang, He. SpyTag/SpyCatcher Cyclization Enhances the Thermostability of Firefly Luciferase.PLoS One. 2016;11(9):e0162318 View Article
  13. Zhang, Wen-Bin; Sun, Fei; Tirrell, David A; Arnold, Frances H. Controlling macromolecular topology with genetically encoded SpyTag-SpyCatcher chemistry.J Am Chem Soc. 2013 09 18;135(37):13988-97 View Article
  14. Fairhead, Michael; Veggiani, Gianluca; Lever, Melissa; Yan, Jun; Mesner, Dejan; Robinson, Carol V; Dushek, Omer; Van der Merwe, P Anton; Howarth, Mark. SpyAvidin hubs enable precise and ultrastable orthogonal nanoassembly.J Am Chem Soc. 2014 09 03;136(35):12355-63 View Article
  15. Tan, Lee Ling; Hoon, Shawn S; Wong, Fong T. Kinetic Controlled Tag-Catcher Interactions for Directed Covalent Protein Assembly.PLoS One. 2016;11(10):e0165074 View Article
  16. Wang, Jindan; Wang, Yilin; Wang, Xinzhe; Zhang, Dandan; Wu, Shuyu; Zhang, Guangya. Enhanced thermal stability of lichenase from Bacillus subtilis 168 by SpyTag/SpyCatcher-mediated spontaneous cyclization.Biotechnol Biofuels. 2016;9:79 View Article
  17. Thrane, Susan; Janitzek, Christoph M; Matondo, Sungwa; Resende, Mafalda; Gustavsson, Tobias; De Jongh, Willem Adriaan; Clemmensen, Stine; Roeffen, Will; Van de Vegte-Bolmer, Marga; Van Gemert, Geert Jan; Sauerwein, Robert; Schiller, John T; Nielsen, Morten A; Theander, Thor G; Salanti, Ali; Sander, Adam F. Bacterial superglue enables easy development of efficient virus-like particle based vaccines.J Nanobiotechnology. 2016 04 27;14:30 View Article
  18. Bedbrook, Claire N; Kato, Mihoko; Ravindra Kumar, Sripriya; Lakshmanan, Anupama; Nath, Ravi D; Sun, Fei; Sternberg, Paul W; Arnold, Frances H; Gradinaru, Viviana. Genetically Encoded Spy Peptide Fusion System to Detect Plasma Membrane-Localized Proteins InA Vivo.Chem Biol. 2015 08 20;22(8):1108-21 View Article
  19. Sun, Fei; Zhang, Wen-Bin; Mahdavi, Alborz; Arnold, Frances H; Tirrell, David A. Synthesis of bioactive protein hydrogels by genetically encoded SpyTag-SpyCatcher chemistry.Proc Natl Acad Sci U S A. 2014 08 05;111(31):11269-74 View Article
  20. Brune, Karl D; Leneghan, Darren B; Brian, Iona J; Ishizuka, Andrew S; Bachmann, Martin F; Draper, Simon J; Biswas, Sumi; Howarth, Mark. Plug-and-Display: decoration of Virus-Like Particles via isopeptide bonds for modular immunization.Sci Rep. 2016 01 19;6:19234 View Article
  21. Veggiani, Gianluca; Nakamura, Tomohiko; Brenner, Michael D; Gayet, RaphaA"l V; Yan, Jun; Robinson, Carol V; Howarth, Mark. Programmable polyproteams built using twin peptide superglues.Proc Natl Acad Sci U S A. 2016 02 02;113(5):1202-7 View Article
  22. Schoene, Christopher; Bennett, S Paul; Howarth, Mark. SpyRing interrogation: analyzing how enzyme resilience can be achieved with phytase and distinct cyclization chemistries.Sci Rep. 2016 02 10;6:21151 View Article
  23. Brune, Karl; Buldun, Can; Li, Yuanyuan; Taylor, Iona; Brod, Florian; Biswas, Sumi; Howarth, Mark. Dual plug-and-display synthetic assembly using orthogonal reactive proteins for twin antigen immunization.Bioconjug Chem. 2017 04 24; View Article
  24. Gao, Xiaoye; Fang, Jie; Xue, Bin; Fu, Linglan; Li, Hongbin. Engineering Protein Hydrogels Using SpyCatcher-SpyTag Chemistry.Biomacromolecules. 2016 09 12;17(9):2812-9 View Article
  25. Schoene, C; Bennett, S P; Howarth, M. SpyRings Declassified: A Blueprint for Using Isopeptide-Mediated Cyclization to Enhance Enzyme Thermal Resilience.Methods Enzymol. 2016;580:149-67 View Article
  26. Botyanszki, Zsofia; Tay, Pei Kun R; Nguyen, Peter Q; Nussbaumer, Martin G; Joshi, Neel S. Engineered catalytic biofilms: Site-specific enzyme immobilization onto E. coli curli nanofibers.Biotechnol Bioeng. 2015 10;112(10):2016-24 View Article
  27. Liu, Zhida; Zhou, Hang; Wang, Wenjun; Tan, Wenjie; Fu, Yang-Xin; Zhu, Mingzhao. A novel method for synthetic vaccine construction based on protein assembly.Sci Rep. 2014 12 01;4:7266 View Article
  28. PrA?schel, Marlene; Detsch, Rainer; Boccaccini, Aldo R; Sonnewald, Uwe. Engineering of Metabolic Pathways by Artificial Enzyme Channels.Front Bioeng Biotechnol. 2015;3:168 View Article
  29. Siegmund, Vanessa; Piater, Birgit; Zakeri, Bijan; Eichhorn, Thomas; Fischer, Frank; Deutsch, Carl; Becker, Stefan; Toleikis, Lars; Hock, BjA?rn; Betz, Ulrich a K; Kolmar, Harald. Spontaneous Isopeptide Bond Formation as a Powerful Tool for Engineering Site-Specific Antibody-Drug Conjugates.Sci Rep. 2016 12 16;6:39291 View Article
  30. Wang, Hejia Henry; Altun, Burcin; Nwe, Kido; Tsourkas, Andrew. Proximity-Based Sortase-Mediated Ligation.Angew Chem Int Ed Engl. 2017 05 02;56(19):5349-5352 View Article
  31. Alves, Nathan J; Turner, Kendrick B; Medintz, Igor L; Walper, Scott A. Protecting enzymatic function through directed packaging into bacterial outer membrane vesicles.Sci Rep. 2016 04 27;6:24866 View Article
  32. Chen, Allen Y; Deng, Zhengtao; Billings, Amanda N; Seker, Urartu O S; Lu, Michelle Y; Citorik, Robert J; Zakeri, Bijan; Lu, Timothy K. Synthesis and patterning of tunable multiscale materials with engineered cells.Nat Mater. 2014 05;13(5):515-23 View Article
  33. Regan, Lynne; Caballero, Diego; Hinrichsen, Michael R; Virrueta, Alejandro; Williams, Danielle M; O?hern, Corey S. Protein design: Past, present, and future.Biopolymers. 2015 07;104(4):334-50 View Article
  34. Leonard, John D; Narlikar, Geeta J. A nucleotide-driven switch regulates flanking DNA length sensing by a dimeric chromatin remodeler.Mol Cell. 2015 03 05;57(5):850-9 View Article
  35. Liu, Xueliang; Lopez, Paola A; Giessen, Tobias W; Giles, Michael; Way, Jeffrey C; Silver, Pamela A. Engineering Genetically-Encoded Mineralization and Magnetism via Directed Evolution.Sci Rep. 2016 11 29;6:38019 View Article
  36. Schmid-Burgk, Jonathan L; HA?ning, Klara; Ebert, Thomas S; Hornung, Veit. CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism.Nat Commun. 2016 07 28;7:12338 View Article
  37. Zakeri, Bijan; Lu, Timothy K. Synthetic biology of antimicrobial discovery.ACS Synth Biol. 2013 07 19;2(7):358-72 View Article
  38. Alam MK, El-Sayed A, Barreto K, Bernhard W, Fonge H, Geyer CR. Site-Specific Fluorescent Labeling of Antibodies and Diabodies Using SpyTag/SpyCatcher System for In Vivo Optical Imaging. Mol Imaging Biol. 2018 Jun 12. View Article

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KERAFAST, INC.
KERAFAST, INC.
KERAFAST, INC.
An Absolute Biotech Company
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Shirley MA 01464
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