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Product Name | Human Latent TGF-beta1 ELISA Kit |
Description | The human latent TGF-β1 ELISA Pro kit provides all necessary reagents and solutions required to quantify human latent TGF-β1 in solution, e.g. in cell culture supernatant, and serum/plasma samples. The kit contains ELISA plates pre-coated with monoclonal capture antibody, biotinylated detection antibody, Streptavidin-HRP, TMB substrate, ELISA standard and ELISA diluent. The ELISA diluent prevents false positive read-outs which may be caused by heterophilic antibodies found in serum and plasma samples. TGF-β1 is secreted in a latent, biologically inactive form, non-covalently linked with a Latency Associated Protein (LAP). The monoclonal antibodies MT593 and MT517-biotin bind to the LAP entity of human latent TGF-β1. This enables direct quantification of latent TGF-β1 in ELISA with no need of sample pre-treatment. For quantification of latent TGF-β1 in blood, the use of plasma is recommended since serum contains high levels of latent TGF-β1 released from platelets during sample preparation. Plasma can be obtained using EDTA, citrate, or heparin as anti-coagulants. To minimize the platelet conten in the sample, and additional centrifugation of plasma at 10,000 x g for 10 minutes is recommended. The ELISA does not detect human latent TGF-β2 or -β3 or bovine latent TGF-β1. Range: 0.4-40 pM |
Size | 2 plates |
Concentration | n/a |
Applications | ELISA |
Other Names | EIA, assay, enzyme immunoassay, test |
Gene, Accession, CAS # | Gene ID: 7040 |
Catalog # | 3550-1HP-2 |
Price | $675 |
Order / More Info | Human Latent TGF-beta1 ELISA Kit from MABTECH AB |
Product Specific References | n/a |