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Product Name | Human IFN-gamma/IL-4/IL-5 FluoroSpot Kit |
Description | The FluoroSpot Plus assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes, which is a major advantage. The human IFN-γ/IL-4/IL-5 FluoroSpot kit is perfect for users who wish to have a convenient assay with minimized assay variability. It enables enumeration of cells secreting one, two or three analytes. The kit includes low fluorescent plates pre-coated with monoclonal capture antibodies, monoclonal detection antibodies, secondary reagents conjugated to fluorophores, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation. A polyclonal activator for human T cells (mAb CD3-2) is supplied as a positive control for cytokine secretion. For analysis, we recommend the use of an automated FluoroSpot reader equipped with filters for excitation 490 nm/emission 510 nm (FITC) for 490-reagent detection, excitation 550 nm/emission 570 nm (Cy3) for 550-reagent detection and excitation 640 nm/ emission 660 nm (Cy5) for 640-reagent detection. Filters should have high specificity to avoid bleed-through artifacts. |
Size | 2 plates |
Concentration | n/a |
Applications | FluoroSpot |
Other Names | interferon/interleukin EIA, assay, enzyme immunoassay, test |
Gene, Accession, CAS # | Gene ID: 3458, 3565, 3567 |
Catalog # | FSP-011608-2 |
Price | $1135 |
Order / More Info | Human IFN-gamma/IL-4/IL-5 FluoroSpot Kit from MABTECH AB |
Product Specific References | PubMed: 30891032, 32313102, 33674735, 26718687, 28542586 |