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Product Name | 2X SDS-PAGE Sample Buffer without DTT or b-ME |
Description | SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearize proteins and to impart a negative charge to linearized proteins. Visit our newly expanded web site at www.rockland.com for methods using this and other buffers. |
Size | 100 mL |
Concentration | 2X |
Applications | 2X SDS-PAGE Sample Loading Buffer is suitable for laboratory assays involved in protein biochemistry. 2X SDS-PAGE Sample Buffer is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 2X SDS-PAGE Sample Buffer consists of 0.125 M Tris, 4% (w/v) SDS, 20% (v/v) Glycerol and 0.01% (w/v) bromophenol blue. This product is not reduced and does not contain DTT or b-ME. The pH of this solution is 6.8. It was meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water. |
Other Names | Sample buffer for protein electrophoresis, Sodium dodecyl sulfate polyacrylamide sample buffer, Protein Gel Sample Buffer, polyacrylamide gel sample buffer, Lysate sample buffer, 2X Lysate Buffer |
Gene, Accession, CAS # | n/a |
Catalog # | MB-018 |
Price | $86 |
Order / More Info | 2X SDS-PAGE Sample Buffer without DTT or b-ME from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | PubMed: 20670848 // 25332149 // 26518752 // 28495207 // 29858079 // 31134659 // 31466866 // 32295130 // 34611843 // 34679020 // 34713936 // 34991629 // 35182754 // 35805580 |