Rabbit TrueBlot® Western Blot Kit from ROCKLAND IMMUNOCHEMICALS INC.
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| Product Name | Rabbit TrueBlot® Western Blot Kit |
| Description | Rabbit TrueBlot® Antibody Peroxidase Conjugate was prepared from tissue culture supernatant by Protein G affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against Anti-Goat Serum. Reactivity is observed against native Rabbit IgG by both Western blot and ELISA. The Rabbit TrueBlot® Western Blot Kit contains the critical supporting reagents, buffers, and substrates for immunoprecipitation and Western blotting of samples using TrueBlot monoclonal secondary antibody in conjunction with your own primary IP antibody and primary (Rabbit IgG) Western blotting antibody (see application note for kit components). Rabbit IgG TrueBlot® is the unique horseradish peroxidase conjugated Anti-Rabbit IgG monoclonal secondary antibody which enables detection of immunoblotted target protein bands, without hindrance by interfering immunoglobulin heavy and light chains from your IP antibody. Use it in place of your conventional HRP Anti-Rabbit IgG immunoblotting secondary antibody. It is easy to generate publication-quality IP/WB data with Rabbit IgG TrueBlot®. Rabbit IgG TrueBlot is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of rabbit IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Rabbit IgG TrueBlot with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/immunoblot applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions. Rabbit IgG TrueBlot may also be used for detection in immunoblotting assays that do not employ immunoprecipitation. |
| Size | 1 kit |
| Concentration | n/a |
| Applications | Immunoprecipitation, Western Blot |
| Other Names | Anti-Rabbit IgG HRP, TrueBlot, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting, TrueBlot for chemiluminescent western blotting |
| Gene, Accession, CAS # | n/a |
| Catalog # | 88-8886-31 |
| Price | $349 |
| Order / More Info | Rabbit TrueBlot® Western Blot Kit from ROCKLAND IMMUNOCHEMICALS INC. |
| Product Specific References | PubMed: 32359397 // 36898832 |
ROCKLAND IMMUNOCHEMICALS INC.
ROCKLAND IMMUNOCHEMICALS INC.
PO Box 5199
Limerick PA 19468
P: 484-791-3823
P: 800.656.7625
F: 484.369.8654
orders@rockland.com
Technical Support: tech@rockland.com
https://www.rockland.com/
Profile of ROCKLAND IMMUNOCHEMICALS INC.