Fasciclin III (Drosophila) Antibody from DEVELOPMENTAL STUDIES HYBRIDOMA BANK
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| Antigenic Specificity | Fasciclin III (Drosophila) |
| Clone | 7G10 anti-Fasciclin III |
| Host Species | Mouse |
| Reactive Species | Drosophila |
| Isotype | IgG2a |
| Format | supernatant |
| Size | 1 ml |
| Concentration | n/a |
| Applications | Immunofluorescence,Immunohistochemistry,Immunoprecipitation |
| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
| Description | Fas III is expressed on a subset of neurons and axons in the CNS, patches of ectoderm, ectodermal stripes, parts of the hindgut and foregut, visceral mesoderm, and the salivary gland. The antibody can be used to stain dissections without detergent as the epitope is on the extracellular domain of the protein. After 10 hrs in development, ectodermal staining will obscure CNS in whole mount preps and therefore dissected preparations are recommended for embryos staining past this age. During larval development, staining is present on a subset of neurons, the epithelial layer of the discs and a cyclone fence pattern in the eye disc. It is especially useful for following the development of the RP1 and RP3 neurons. The antibody works for affinity purification but does not work for immunoprecipitation or western. This antibody may work for Immunoprecipitation. While the Depositor clearly stated that it doesn't work for immunoprecipitation , the Initial Publication, in which the Depositor is the last author, reports the succesful use of 7G10 for the immunoprecipitation of Fasciclin III. Antibody Registry ID: AB_528238 |
| Immunogen | Membrane preparation from nerve cord and cultures of primary neurons (10-13 hr development). |
| Other Names | Fasciclin 3 |
| Gene, Accession # | Fas3, Gene ID: 35097, UniProt: P15278 |
| Catalog # | 7G10 anti-Fasciclin III |
| Price | please inquire |
| Order / More Info | Fasciclin III (Drosophila) Antibody from DEVELOPMENTAL STUDIES HYBRIDOMA BANK |
| Product Specific References | PubMed: 3548998 |
DEVELOPMENTAL STUDIES HYBRIDOMA BANK
DEVELOPMENTAL STUDIES HYBRIDOMA BANK
Dr. David Soll
University of Iowa Dept. Biology
Iowa City IA 52242
P: 319-335-3826
F: 319-335-2077
dshb@uiowa.edu
http://dshb.biology.uiowa.edu
Profile of DEVELOPMENTAL STUDIES HYBRIDOMA BANK