ATPase, (Na(+) K(+)) alpha-1 subunit Antibody from DEVELOPMENTAL STUDIES HYBRIDOMA BANK
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| Antigenic Specificity | ATPase, (Na(+) K(+)) alpha-1 subunit |
| Clone | a6F |
| Host Species | Mouse |
| Reactive Species | avian, dog, fish, human, mouse, porcine, rat, Torpedo, zebrafish |
| Isotype | IgG2a, kappa |
| Format | HYBRIDOMA |
| Size | 1 vial cells |
| Concentration | n/a |
| Applications | ELISA,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot |
| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
| Description | Epitope: N-terminal region between aa 27-55. In the 160 aa near the N-terminus, probably between aa 34-52 [PMID 8798546]. Recognizes a cytosolic epitope on the alpha-subunit of the Na, K-ATPase, alpha-1 isoform. The epitope appears to be linear, and located on the N-terminal region between aa 27-55. Weak or no binding to sheep and Xenopus. Works great for Immunoblotting. Immunochemistry: good on cryosections; poor for lightly-fixed cells in tissue culture; seems to require denaturation of Na, K-ATPase. This hybridoma was made together with alpha-7C, described in [PMID 2831227]. Broad species specificity [PMID 8798546]. This antibody doesn't appear to work on conventionally fixed tissue, and stains fixed sections of the rat kidney faintly, but this staining is considerably increased by prior treatment of the sections with SDS [PMID 8798546]. RRID: AB_528092 |
| Immunogen | purified adult chicken kidney (Na+, K+)-ATPase alpha-subunit., Total protein |
| Other Names | 6F; alpha6F; Atp1a1 |
| Gene, Accession # | ATP1A1, Gene ID: 396530, UniProt: P09572 |
| Catalog # | a6F |
| Price | please inquire |
| Order / More Info | ATPase, (Na(+) K(+)) alpha-1 subunit Antibody from DEVELOPMENTAL STUDIES HYBRIDOMA BANK |
| Product Specific References | PubMed: 2831227 |
DEVELOPMENTAL STUDIES HYBRIDOMA BANK
DEVELOPMENTAL STUDIES HYBRIDOMA BANK
Dr. David Soll
University of Iowa Dept. Biology
Iowa City IA 52242
P: 319-335-3826
F: 319-335-2077
dshb@uiowa.edu
http://dshb.biology.uiowa.edu
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