cadherin, DN- (extracellular domain) Antibody from DEVELOPMENTAL STUDIES HYBRIDOMA BANK
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| Antigenic Specificity | cadherin, DN- (extracellular domain) |
| Clone | DN-Ex #8 |
| Host Species | Rat |
| Reactive Species | Drosophila |
| Isotype | IgG2a |
| Format | supernatant |
| Size | 1 ml |
| Concentration | n/a |
| Applications | Immunofluorescence,Immunohistochemistry,Western Blot |
| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
| Description | Epitope: extracellular region including CR2-CR8. Suitable to use in immunostaining and western blots. 1/ Staining of embryos and discs; both fixative 1 (3.7% formaldehyde in PBS) and fixative 2 (4% paraformaldehyde in CMF & GBS containing 1mM CaCl2 (see details in Dev. Biol. 165, 716-726)) work. 2/ Staining of S2 cells; fixative 1 doesn't work, use fixative 2. 3/ Staining of pupal or adult heads for light microscope; find details in our MCN paper and references therein such as Uchida et al. (4% paraformaldehyde-based fixative on ice for 1-2 hr) and Hoshino et al. (PLP-based fixative on ice for 1-2 hr). For better fixation, we cut pupal or adult heads into halves. 4? ImmunoEM of adult heads; find details in our MCN paper. We do not add EDTA or EGTA in any fixatives, because cadherins are easily degraded under Ca++- free conditions. Marker of neuropil. Antibody Registry ID: AB_528121 |
| Immunogen | Recombinant fusion protein containing the extracellular region of N-cadherin from cadherin repeats 2 to 8., Partial protein |
| Other Names | CG7100, N-cadh |
| Gene, Accession # | CadN, Gene ID: 35070, UniProt: O15943 |
| Catalog # | DN-Ex #8 |
| Price | please inquire |
| Order / More Info | cadherin, DN- (extracellular domain) Antibody from DEVELOPMENTAL STUDIES HYBRIDOMA BANK |
| Product Specific References | PubMed: 9247265 |
DEVELOPMENTAL STUDIES HYBRIDOMA BANK
DEVELOPMENTAL STUDIES HYBRIDOMA BANK
Dr. David Soll
University of Iowa Dept. Biology
Iowa City IA 52242
P: 319-335-3826
F: 319-335-2077
dshb@uiowa.edu
http://dshb.biology.uiowa.edu
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