CBP bromodomain TR-FRET Assay Kit from CAYMAN CHEMICAL COMPANY
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| Product Name | CBP bromodomain TR-FRET Assay Kit |
| Description | Cayman's CBP bromodomain TR-FRET Assay Kit is a homogeneous, time-resolved Foerster resonance energy transfer (TR-FRET) assay method amenable to rapid characterization of inhibitors of the bromodomain-acetylated peptide interaction in a high-throughput format. The donor fluorophore in this assay consists of CBP bromodomain (BRD; human; amino acids 1,081-1,197) directly labeled with a europium (Eu3+) chelate (BRD-Eu3+). A biotinylated peptide containing acetylated lysines serves as the ligand for the CBP bromodomain. Allophycocyanin (APC)-labeled avidin binds with high affinity to the peptide substrate via the biotin moiety and serves as the acceptor fluorophore in the assay. Inhibition of the BRD-peptide interaction displaces BRD-Eu3+ from the APC-labeled avidin resulting in a loss of TR-FRET signal. The CBP bromodomain TR-FRET Assay Kit is robust (Z? = >0.6) and suitable for high-throughput screening in the provided 384-well plate or can be scaled to higher density plate formats (e.g., 1,536-well plate) if desired. The assay is stable at room temperature for at least four hours and in the presence of less than 2% DMSO. |
| Size | 384 Well |
| Concentration | n/a |
| Applications | Fluorescence, High-Throughput Screening, TR-FRET |
| Other Names | TRFRET BRDEu BRDEu3+ HTS |
| Gene, Accession, CAS # | n/a |
| Catalog # | 600850-384WELLS |
| Price | $574.00 |
| Order / More Info | CBP bromodomain TR-FRET Assay Kit from CAYMAN CHEMICAL COMPANY |
| Product Specific References | n/a |
CAYMAN CHEMICAL COMPANY
CAYMAN CHEMICAL COMPANY
1180 East Ellsworth Road
Ann Arbor MI 48108
P: (734) 971-3335
P: (800) 364-9897 (USA & Canada only)
F: (734) 971-3640
Customer Service: custserv@caymanchem.com
Technical Support: techserv@caymanchem.com
Sales: sales@caymanchem.com
http://www.caymanchem.com
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