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Product Name | SIRT2 Direct Fluorescent Screening Assay Kit |
Description | The sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs) that catalyze a reaction coupling lysine deacetylation to the formation of nicotinamide and O-acetyl-ADP-ribose. Cayman's Direct Fluorescent Screening Assay Kits provide a convenient fluorescence-based method for screening SIRT inhibitors or activators. The procedure requires only two easy steps, both performed in the same microplate. In the first step, the substrate is incubated with human recombinant SIRT along with its cosubstrate NAD+. Deacetylation sensitizes the substrate such that treatment with the developer in the second step releases a fluorescent product. The fluorophore can be analyzed with an excitation wavelength of 350-360 nm and an emission wavelength of 450-465 nm. |
Size | 96 Well |
Concentration | n/a |
Applications | Fluorescence |
Other Names | Nucleosomes chromosomal deoxyribonucleases DNA histones H2A H2B H3 H4 octameric cores transcription amino termini acetylation phosphorylation methylation ε-amino lysines acetyltransferases HATs chromatins genes activation deacetylases HDACs catalyzes hydrolytic acetyls sirtuins trichostatins A-insensitive lysyl-deacetylases class III catalyzes deacetylation nicotinamides O-acetyl-ADP-ribose NAD+ human SIRT1 SIRT2 SIRT3 SIRT4 SIRT5 SIRT6 SIRT7 cytoplasmic proteins H4 lysine-16 lysine-40 α-tubulin a-tubulin alpha-tubulin HDAC6 microtubules mitotic chromosomal hyperpolid cells tissues skeletal muscle heart oligodendroglia brain tumor suppressor gliomas Down-regulation gene chromosomal region diagnostic marker fluorescence-based inhibitors activators p53 Gln-Pro-Lys-Lys(ε-acetyl)-AMC recombinant NAD+ fluorescent fluorescence kits assays measures measurement |
Gene, Accession, CAS # | n/a |
Catalog # | 700280-96WELLS |
Price | $348.00 |
Order / More Info | SIRT2 Direct Fluorescent Screening Assay Kit from CAYMAN CHEMICAL COMPANY |
Product Specific References | n/a |